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 IBD 
CRECER
CRECER Ozone FACES
Research : Biomarkers of Ozone Exposure and Air Pollution

Biomarkers in Children and Environmental Pollution
 Collaborators 

University of California, San Francisco
    John Balmes, M.D.
    Mehrdad Arjomandi, M.D.

University of California, Berkeley
    Ira Tager, M.D.
    Subha Venkat, PhD
    Bobby Dhesi
    Aurelia Cheng

    This study focuses on the health effects of healthy individuals to ambient levels of tropospheric ozone (O3), a potent oxidant and outdoor pollutant. More stringent air pollution legislation has contributed to a downward trend in air pollution levels over the last two decades. However, approximately 100 million Americans still reside in areas that exceed the new 8-hour U.S. O3 standard of 80 ppb (3 year average of 4th highest). Primary injury is caused by ozone is related to ozone's effects on cell membranes and involve lipid peroxidation. Chronic exposure has been associated with decreased levels of lung function in children and adolescents. The main study investigates the effects of long-term exposure to O3 on lung function in a group of college freshmen. The cohort consists of students who were lifelong residents of the Los Angeles and San Francisco Bay area. A sub-cohort of these students participated in an acute-O3 study where they were exposed to 200 ppb O3 in a chamber.

    The goals of this study include: 1) Determining the effect of short-term and long-term exposure to varying levels of ambient ozone on cytogenetic damage using the micronucleus (MN) assay, DNA damage by the COMET assay, 2) Assessing the importance of various markers of lipid peroxidation (malondialdehyde, 8-isoprostane), antioxidant status (FRAP) and epithelial injury (CC16), and 3) Assessing the role of polymorphisms in genes involved in antioxidant defense (i.e. GST M1, GST P1, SOD, GPX, NQO1).
 Publications

Arjomandi, M., Tager, et al (2008). "Is there an association between lifetime cumulative exposure and acute pulmonary responses to ozone." JOEM 50(3): 341-9.

Chen C, Arjomandi M, et al (2007). "Effects of Chronic and Acute Ozone Exposure on Lipid Peroxidation and Antioxidant Capacity in Healthy Young Adults." Environmental Health Perspective. 2007 Dec;115(12):1732-7.

Tager, I. B., J. Balmes, et al. (2005). "Chronic exposure to ambient ozone and lung function in young adults." Epidemiology 16(6): 751-9.

Tujague, J., M. Bastaki, et al. (2006). "Antioxidant intake, GSTM1 polymorphism and pulmonary function in healthy young adults." Eur Respir J 27(2): 282-8.

Chen C, Arjomandi M, Qin H, Balmes J, Tager I, Holland NT. Cytogenetic Damage in Buccal Epithelia and Peripheral Lymphocytes in Young Healthy Individuals Exposed to Ozone. Mutagenesis. 21:131-137, 2006.

Huen K, Gunn L, Duramad P, Jeng M, Scalf R, Holland NT. Application of a Geographic Information System to Explore Associations Between Air Pollution and Cytogenetic Damage in African-American Children and Adults. Environmental Molecular Mutagenesis. 47:236-246, 2006.

M. Neri, D Ugolini, S Bonassi,  A Fucic, N Holland, LE Knudsen, R J Sram, M Ceppi,V. Bocchini, DF Merlo.Children’s exposure to environmental pollutants and biomarkers of genetic damage: I Overview and critical issues. Mutation Research Reviews, 612:1-13, 2006.

M. Neri, D. Ugolini, S. Bonassi, A. Fucic, N. Holland, L. Knudsen, R. Sram, M. Ceppi, Vittorio Bocchini, and Domenico Franco Merlo. Children’s exposure to environmental pollutants and biomarkers of genetic damage: II. Results of a comprehensive literature search, Mutation Research Reviews, 612:14-39, 2006.

R Aamodt, A.Anouna, P.Baird, J.Beck, m.Bledsoe, Y De Souza, W.Grizzle, J.Gosh, N.Holland, J.Vaught.  ISBER (International Society for Biological and Environmental Repositories), Best practices for repositories I:  Collection, storage, and retrieval of human biological materials for research.  Cell Preservation and Technology, 3(1):5-48, 2005.

Holland NT, Pfleger LP, Berger E, Ho A, Bastaki M. Molecular Epidemiology Biomarkers - Sample Collection and Banking Considerations. Toxicology and Applied Pharmacology, 206:261-68, 2005.

Bastaki, M., K. Huen, et al. "Genotype-activity relationship for Mn-superoxide dismutase (MnSOD), glutathione peroxidase 1 (GPX1) and catalase in humans." Pharmacogenetics and Genomics, 2006 Apr;16(4):279-86.

M.Fenech, S.Bonassi, J.Turner, M.Ceppi, W.P. Chang, N.Holland et al.”Intra- and interlaboratory variation in the scoring of micronuclei and nucleoplasmic bridges in binucleated human lymphocytes. Results of an international slide-scoring  exercise by the HUMN project” Mutation Research, 534/1-2: 45-64, 2003.

M.Fenech, WP Chang, M.Kirsch-Volders, N.Holland, S.Bonassi, E.Zeiger “HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures”, MutationResearch, 534: 65-75, 2003.

Holland NT, Zhang L, Smith MT. "Cytogenetics biomarkers and air pollution", Relationship Between Acute and Chronic Effects of Air Pollution, ed. U. Heinrich & U. Mohr, ILSI Press, Washington, D.C. pp. 65-78, 2000.

Kunzli, N., F. Lurmann, et al. (1997). "Association between lifetime ambient ozone exposure and pulmonary function in college freshmen--results of a pilot study." Environ Res 72(1): 8-23.


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