[full text] [abstract]
Published
Environmental and Molecular Mutagenesis 37:31-45 (2001)
Title
HUman MicroNucleus project: International Database Comparison for Results With the Cytokinesis-Block Micronucleus Assay in Human Lymphocytes: I. Effect of laboratory protocol, scoring criteria, and host factors on the frequency of micronuclei.
Authors
Stefano Bonassi1*, Michael Fenech2, Cecilia Lando1, Yi-ping Lin3, Marcello Ceppi1, Wushou Peter Chang3, Nina Holland4, Micheline Kirsch-Volders5, Errol Zeiger6, Sadayuki Ban7, Roberto Barale8, Maria Paola Bigatti9, Claudia Bolognesi10, Cao Jia11, Marina Di Giorgio12, Lynnette R. Ferguson13, Aleksandra Fucic14, Omar Garcia Lima15, Patrizia Hrelia16, Ayyathan P. Krishnaja17, Tung-Kwang Lee18, Lucia Migliore8, Ludmilla Mikhalevich19, Ekaterina Mirkova20, Pasquale Mosesso21, Wolfgang-Ulrich Müller22, Youichi Odagiri23, Maria Rosaria Scarffi24, Elena Szabova25, Irena Vorobtsova26, Anne Vral27, Andrea Zijno28
Organizations
1Department of Environmental Epidemiology,
Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy
2Commonwealth Scientific and Industrial
Research Organization (CSIRO) Health Sciences and Nutrition,
Adelaide, Australia
3Institute of Environmental Health
Sciences, National Yang Ming University Medical School, Taipei,
Taiwan, Republic of China
4School of Public Health, University
of California, Berkeley, California
5Laboratory for Cell Genetics,
Vrije Universiteit Brussels, Brussels, Belgium
6Environmental Toxicology Program,
National Institute of Environmental Health Sciences, Research
Triangle Park, North Carolina
7Department of Radiobiology, Radiation
Effects Research Foundation, Hiroshima, Japan
8Dipartimento di Scienze dell'Uomo
e dell'Ambiente, University of Pisa, Pisa, Italy
9Dipartimento di Biologia Animale,
University of Turin, Turin, Italy
10Toxicological Evaluation Unit,
Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy
11Molecular Toxicology Laboratory,
Third Military Medical University, Chong Qing, China
12Laboratorio Dosimetria Biologica,
Autoridad Regulatoria Nuclear, Buenos Aires, Argentina
13Auckland Cancer Society Research
Centre, Auckland, New Zealand
14Institute for Medical Research
end Occupational Health, Zagreb, Croatia
15Centro de Proteccion e Higiene
de las Radiaciones, Havana, Cuba
16Department of Pharmacology, University
of Bologna, Bologna, Italy
17Cell Biology Division, Bhabha
Atomic Research Center, Mumbai, India
18Department of Radiation Oncology,
Leo Jenkins Cancer Center, East Carolina University School
of Medicine, Greenville, North Carolina
19Institute of Genetics and Cytology,
Belarus Academy of Sciences, Minsk, Belarus
20Nacional Center of Hygiene, Medical
Ecology and Nutrition, Sofia, Bulgaria
21Dipartimento di Agrobiologia
e Agrochimica, Università della Tuscia, Viterbo, Italy
22Institute für Medizinische
Strahlenbiologie, Universitatsklinikum Essen, Essen, Germany
23Division of Human and Health
Sciences, Yamanashi Prefectural College of Nursing, Kofu,
Japan
24Consiglio Nazionale delle Ricerche
(CNR)-Istituto Di Ricerca Per l'Elettromagnetismo ei Componendi
Elettronici (IRECE), Naples, Italy
25Institute of Preventive and Clinical
Medicine, Bratislava, Slovak Republic
26Central Research Institute of
Roentgenology and Radiology, St. Petersburg, Russia
27Department of Anatomy, Embryology,
and Histology, University Gent, Ghent, Belgium
28Laboratory of Comparative Toxicology
and Ecotoxicology, Istituto Superiore di Sanità, Rome,
Italy
Full Text
* Full text of this paper can be requested from Dr. Stefano Bonassi, Department of Environmental Epidemiology, Istituto Nazionale per la Ricerca sul Cancro, Largo R. Benzi, 10, I-16132 Genoa Italy (E-mail: bonassi@hp380.ist.unige.it) or any other HUMN committee member (listed on the front page).
As an alternative, the full text may also be downloaded in electronic format from one of the links below:
PDF: HUMN
PDF: InterScience
Abstract
Micronucleus (MN) expression in peripheral blood lymphocytes is well established as a standard method for monitoring chromosome damage in human populations. The first results of an analysis of pooled data from laboratories using the cytokinesis-block micronucleus (CBMN) assay and participating in the HUMN (HUman MicroNucleus project) international collaborative study are presented. The effects of laboratory protocol, scoring criteria, and host factors on baseline micronucleated binucleate cell (MNC) frequency are evaluated, and a reference range of "normal" values against which future studies may be compared is provided. Primary data from historical records were submitted by 25 laboratories distributed in 16 countries. This resulted in a database of nearly 7000 subjects. Potentially significant differences were present in the methods used by participating laboratories, such as in the type of culture medium, the concentration of cytochalasin-B, the percentage of fetal calf serum, and in the culture method. Differences in criteria for scoring micronuclei were also evident. The overall median MNC frequency in nonexposed (i.e., normal) subjects was 6.5 and the interquartile range was between 3 and 12. An increase in MNC frequency with age was evident in all but two laboratories. The effect of gender, although not so evident in all databases, was also present, with females having a 19% higher level of MNC frequency (95% confidence interval: 14-24%). Statistical analyses were performed using random-effects models for correlated data. Our best model, which included exposure to genotoxic factors, host factors, methods, and scoring criteria, explained 75% of the total variance, with the largest contribution attributable to laboratory methods.
Environ. Mol. Mutagen. 37:31-45, 2001
© 2001 Wiley-Liss, Inc.
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