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Title
The HUman MicroNucleus Project--An
international collaborative study on the use of the micronucleus
technique for measuring DNA damage in humans
Authors
Michael Fenecha*, Nina Hollandb, Wushou Peter Changc, Errol Zeigerd, Stefano Bonassie
Organizations
a CSIRO Human Nutrition, Gouger Street, P.O. Box 10041, Adelaide, SA 5000, Australia
b School of Public Health, UniÍersity of California, Berkeley, CA 94720-7360, USA
c Institute of Public Health, National Yang Ming University Medical School, Taipei, Taiwan
d Environmental Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
e Department of Environmental Epidemiology, Instituto Nazionale per la Ricerca sul Cancro, I-16132 Genoa, Italy
Full Text
* Full text of this paper can be requested from Dr. Michael Fenech, CSIRO Human Nutrition, Gouger Street, P.O. Box 10041, Adelaide, SA 5000, Australia (E-mail: michael.fenech@hsn.csiro.au) or any other HUMN committee member (listed on the front page).
The full text may also be downloaded in electronic format from
one of the links below:
PDF: HUMN
PDF: Elsevier
Abstract
The International Collaborative Project on Micronucleus Frequency in Human Populations (HUMN) was organized to collect data on micronucleus (MN) frequencies in different human populations and different cell types. The test procedures considered by this project are assays using human lymphocytes (cytokinesis-block method), exfoliated epithelial cells, and other cell types. Data (including descriptions of the populations monitored, detailed test protocols, and test results) are being obtained from a large number of laboratories throughout the world and are being entered into a unified database. The information will be used to: (1) determine the extent of variation of `normal' values for different laboratories and the influence of other factors potentially affecting baseline MN frequency, e.g., age, gender and life-style; (2) provide information on the effect of experimental protocol variations on MN frequency measurements; (3) design and test optimal protocols for the different cell types; and (4) determine the extent to which MN frequency is a valid biomarker of ageing and risk for diseases such as cancer.
Mutation Research 428:271-283, 1999
© 1999 Elsevier Science B.V. All rights reserved.
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