HUMN Project - Automated Micronucleus Workshop

 
Venue – European Environmental Mutagen Society Meeting – Prague (http://www.eems2006.org/) at the Prague Congress Centre.
Date  - Sunday 2nd July 8.30-12.30

OBJECTIVES

• Review current progress in development of automated systems for measuring micronuclei in human and mammalian cells using image and flow cytometry
• Review current limitations with visual scoring and automated scoring.
• Discuss how consistency can be improved in visual scoring and automated systems.
• Establish criteria for comparing results between visual scoring and automated scoring in order to facilitate the validation process of automated systems
• Explore the possibility of developing a collaborative network focussed on automated systems and their validation as part of the HUMN project

PROGRAM
Chairpersons: Micheline Kirsch-Volders & Michael Fenech

8.30 Michael Fenech: Brief introduction to the HUMN project objectives (10 minutes)

8.40 Roberto Barale:  Health risk predictivity of MN in peripheral lymphocytes and scoring standards  (20 minutes)

9.00 Marlies DeBoeck: Comparison of automated analysis methods for micronucleus assays in human and rodent cells (20 minutes)

9.20 Stephen Dertinger: Flow cytometric methods for scoring micronuclei in erythrocytes and nucleated cells (20 minutes)

9.40  Lilianne Abramsson-Zetterberg: The erythrocyte flow cytometric assay system as a measure of genome damage and nutritional effects in humans (20 minutes)

10.00 Ash Lal: Immunomagnetic separation technique with single-laser flow cytometry to isolate and analyze micronuclei in reticulocytes (20 minutes)

10.20 Zoryana Cammerer: Comparison of the peripheral blood flow cytometric micronucleus test in rat and mouse exposed to aneugens. (10 minutes)

10.30 am Coffee break – 10 minutes

10.40 Christian Schunck: Automated in-vitro and in-vivo micronucleus scoring using the Metafer MicroNuclei slide scanning system (20 minutes)

11.00 Francoise Soussaline: IMSTAR Pathfinder Automated Image Analysis System for integrated imaging measurements and micronuclei detection and scoring (20 minutes)

11.20 Wilfried Frieauff: ROBIAS – fully automated robotic image analysis for micronucleus and other genotox testing in Novartis  (20 minutes)

11.40 Ida-Maria Sintorn : Image analysis for the various novel biomarkers within the cytokinesis-block micronucleus assay (20 minutes)

12.00 Micheline Kirsch-Volders: Advantages and disadvantages of different automated systems and current knowledge gaps (20 minutes)

12.20 Michael Fenech:  Developing a collaborative network focussed on automated systems and their validation as part of the HUMN project (10 minutes).
                                                               
12.30 CLOSE